Fariba Farzam, Jinous Asgarpanah and Arash Mahboubi
Following previous investigations on different species of Tanacetum, as a novel survey, this study was conducted with the aim to evaluation of the antibacterial effect of the total methanol extract Tanacetum frutoculosum Lebeb and its Sub-fractions against 6 bacterial strains including: Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228, Methicillin resistant Staphylococcus aureus ATCC 33591, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027 and Escherichia Coli ATCC 8739. Primarily antibacterial effects were investigated by the Agar well diffusion method and then Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) were determined using broth Macro dilution method. The total phenolic and flavonoid contents of the extract and its fractions were measured based on gallic acid and rutin equivalents (GAE and RE) respectively. The total methanol extract showed the highest phenolic and flavonoid contents (1.858mg GEA/g and 21mg RE/g respectively) and had the highest antibacterial effect against S. epidermidis (MIC 15.62 mg/ml). The petroleum ether fraction showed the lowest phenolic and flavonoid contents (0.760mg GAE/g and 11.6 mg RE/g respectively) and had the lowest antibacterial activity against P. aueroginosa (MIC 250 mg/ml). These findings demonstrated the plant potential antibacterial effect against various human pathogenic bacteria.
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