Balqees Yahya Najm*, Sarab Hussein Khallel, Hala Mahmmed Majeed
The aim of the present study includes the isolation of 25 isolates belonging to the actinomycetes from soil samples collected from different agricultural regions in Baghdad city. The isolates were identified tentatively on the basis of chalky, leathery. Mucoidy, waxy, appearance of colonies. The identification was confirmed by using slide culture technique and observing substrate and aerial mycelium, and arrangement of spore chains. 10 isolates showed capability to produce antimicrobial activity. To more confirm that these isolates were belonged to actinomycetes, they were identified by molecular method using 16rDNA they were obtained a specific band of about 1500 bp the selected isolates were studied for their antimicrobial activity against gram positive and gram negative bacteria. Also study conducted studying the influence of factors such carbohydrate (glucose, maltose, sucrose, lactose and starch) and different concentration of (NH4)2SO4 as nitrogen source on antimicrobial production.
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