Europäisches Journal für Experimentalbiologie Offener Zugang

Abstrakt

N-nitrosation of N-methylaniline and nitrosamine toxicology in the wistar rats

Ehigbor F. Abebe, Comfort J. Akpabio and Emmanuel N. Maduagwu

The aim of this study is to investigate the urinary excretion of nitrosamine ions in rats, to investigate the effect of precursors of N-nitrosamine on serum enzymes activities and to evaluate the toxicity on liver on exposure to Nnitrosamine precursors. Forty two male wistar rats were divided into six groups and each group was admininistered with varying doses of sodium nitrite and N-methylaniline. The following methods were used; spectrophotometry, thin layer chromatography, cell fractionation, centrifugation, dialysis, histopathology, enzymatic and colorimetry. The RF values of the test samples were similar to those of the test standard, signifying the presence of N-nitrosamine in the urine of the rat. Liver function test, showed significant elevations (P < 0.05) in the AST, ALT, ALP and GGT activities in all the test groups compared with the control. Histological evaluation showed severe haemorrhage within the sinusoid, the portal triad was infiltrated by inflammatory cells in both NaNO₂ induced group and NMA combined with NaNO₂ group, thus showing acute damage compared with the control group which showed no lesion. The present study showed the urinary excretion of nitrosamine formed from N-methylaniline and sodium nitrite, the effect of N-methylaniline and sodium nitrite on serum enzymes and their toxicity on the liver of wistar rats.