Europäisches Journal für Experimentalbiologie Offener Zugang

Abstrakt

Prevalence of Anopheles Gambiae S. S and their pyrethroid knock down resistance pattern in five selected communities in kumasi metropolis using polymerase chain reaction (PCR)

N. Agyepong, E. E. Mak-Mensah and C. A. Brown

The Anopheles species play a central role in malaria transmission. Several methods are available to map out the prevalence of various species of the Anopheles and the knock down resistance in the tropics and sub-tropical regions of the world. PCR is faster in determining genetic diversity of these species. This study was set out to identify and determine the prevalence and the pyrethroid knock down resistance pattern of Anopheles gambiae s.s, (the most prevalent malaria vector) within the selected communities in Kumasi. DNA was extracted from Anopheles mosquitos’ larvae and used as template in polymerase chain reaction (PCR), for each specimen. Seventy six out of 100 larvae collected in all the sites were identified as gambiae s.s. Twenty six out of 50 identified An. gambiae s.s were found to posses Kdr gene with remaining 24 samples being susceptible. PCR was successfully used for the identification and prevalence of An. gambiae s.s and determination of their pyrethroid knock down resistance.