Europäisches Journal für Experimentalbiologie Offener Zugang

Abstrakt

Quantification of Gentamicin by microbial assay technique and reverse phase HPLC

Gopalan Arun Kumar and Venkatachalam Ramya

The preliminary research work carried to find out the concentration of Gentamicin in the raw material. Two different techniques used in this study for the quantification of Gentamicin. They are microbiological assay method and reversed phase HPLC. The concentration of Gentamicin was quantified even at very lower concentration by microbiological assay method and it is sensitive. The test organisms used is Staphylococcus epidermidis and Bacillus pumilus. The components present in Gentamicin were estimated by reverse phase HPLC was found to be C1, C1a, C2a and C2. It is therefore concluded that the microbial assay used for assay purpose and HPLC is used for estimating the components present in Gentamicin. This study can be further carried out for the development of Gentamicin by HPLC method as a prolonged research work.