Europäisches Journal für Experimentalbiologie Offener Zugang

Abstrakt

The enzymatic and non-enzymatic antioxidant activities of Arbutus andrachne L. leaf and flower and its antibacterial activities against mastitis pathogens

Nuray Ergun, GultenOkmen, Hikmet Yolcu, Zafer Cantekin, Yasar Ergun, Dilek Isık and Pelin Sengul

For solving the antibiotic resistance problem it needs to explore new antimicrobial substances. Plants can be good sources for these purposes. This study was planned to evaluate the antibacterial and antioxidant activities of Arbutus andrachne leaf and flower. The antibacterial and antioxidant activities were conducted for methanolic extracts of the plant using conventional methods. Antioxidant activity was analyzed using the DPPH free radical scavenging method and high antioxidant activity was found (81%), reached up to 2.10 and 2.11 mmol Trolox equivalent (mM (TE) /g dry biomass), respectively. It was determined that the activities of catalase (CAT) and ascorbate peroxidase (APX) enzymes were higher at the flowers of Arbutus andrachne L. compared to the leaves. The extracts were screened for its antibacterial activity against seven mastitis pathogens. The methanol extract of Arbutus andrachne showed minimal inhibitory activity (MIC) at 3250 μg/mL. It can thus be concluded that A. andrachne is a good source of antibiotics and antioxidants.