Kirill V Ermakov, Alexander A Bukhvostov, Alexander S Vedenkin, Sergey V Stovbun, Anton S Dvornikov, Anna V Semenova, Dmitry A Kuznetsov.
A significant population of ultrashort (50n-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng ml-1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion-anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.
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